EdRox is a Marie Curie Research Training Network which is committed to provide state-of-the-art training in bio-nanotechnology to early stage researchers. They will be engaged in the development and implementation of the FluoRox principle (a method for fluorometric detection of redox-enzyme activity) into a working prototype of a FluoRox sensor. It includes the engineering and the fluorimetric and electrochemical characterization of functionalized electrodes. The project comprises four steps, largely in parallel:
- selection/adaptation of proteins and enzymes;
- immobilisation of proteins/enzymes;
- enzymatic characterization of the electrodes;
- construction of a working FluoRox sensor.
Research and Training Objectives
The goal of the EdRox research training network is to provide advanced and in depth training in bio-nanotechnology to early stage researchers (ESR's) and "experienced researchers" (ER's). The training program is intimately connected with the research program. The latter focuses on the implementation of a novel concept called FluoRox? to monitor redox reactions in vivo and in vitro by fluorescence detection. Fluorox devices allow detection levels that are orders of magnitude lower than provided by conventional electrochemical methods. They can be used in medical and environmental monitoring. Its implementation requires engineering of existing redox enzymes, linking them to electrodes, incorporating these into an optical detection scheme, and testing them.
Training and research include instruction in state-of-the-art techniques in biophysics, chemistry, biochemistry and biology, and teaching of complementary skills like communication and presentation, research management, handling of intellectual property (IP), teaching, entrepreneurship, and exploration of career opportunities. Educational programs and goals, including personal career development plans, are individually tailored for each ESR and ER fellow in the network and training is divided over at least two laboratories for each fellow.